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A and B show two different interactive regions acquired automatically by multiple-frequency domain <t>FLIM</t> (512x512 frame). The heat map indicates the Ef (the extent of interaction) represented by the pseudo-colour scale. A and B both show that the high expression levels of PD1, (grey scale) do not correlate with high Ef. The quantification of the Ef values is represented by violin plots next to each Ef mapped image. The heterogenous distribution of the interactive data points (per pixel) is shown by the shape of the violin plots. The dotted line at 4% Ef represents the Förster Radius (5.83nm) where there is no PD1-PDL1 interaction. PD1-PDL1 interactions are above 4% Ef.
Multiple Frequency Domain Fluorescence Lifetime Imaging Microscope, supplied by Lambert Instruments BV, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A and B show two different interactive regions acquired automatically by multiple-frequency domain <t>FLIM</t> (512x512 frame). The heat map indicates the Ef (the extent of interaction) represented by the pseudo-colour scale. A and B both show that the high expression levels of PD1, (grey scale) do not correlate with high Ef. The quantification of the Ef values is represented by violin plots next to each Ef mapped image. The heterogenous distribution of the interactive data points (per pixel) is shown by the shape of the violin plots. The dotted line at 4% Ef represents the Förster Radius (5.83nm) where there is no PD1-PDL1 interaction. PD1-PDL1 interactions are above 4% Ef.
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A and B show two different interactive regions acquired automatically by multiple-frequency domain <t>FLIM</t> (512x512 frame). The heat map indicates the Ef (the extent of interaction) represented by the pseudo-colour scale. A and B both show that the high expression levels of PD1, (grey scale) do not correlate with high Ef. The quantification of the Ef values is represented by violin plots next to each Ef mapped image. The heterogenous distribution of the interactive data points (per pixel) is shown by the shape of the violin plots. The dotted line at 4% Ef represents the Förster Radius (5.83nm) where there is no PD1-PDL1 interaction. PD1-PDL1 interactions are above 4% Ef.
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A and B show two different interactive regions acquired automatically by multiple-frequency domain <t>FLIM</t> (512x512 frame). The heat map indicates the Ef (the extent of interaction) represented by the pseudo-colour scale. A and B both show that the high expression levels of PD1, (grey scale) do not correlate with high Ef. The quantification of the Ef values is represented by violin plots next to each Ef mapped image. The heterogenous distribution of the interactive data points (per pixel) is shown by the shape of the violin plots. The dotted line at 4% Ef represents the Förster Radius (5.83nm) where there is no PD1-PDL1 interaction. PD1-PDL1 interactions are above 4% Ef.
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fluorescence lifetime imaging microscope acton sp2558  (Princeton Instruments)
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Princeton Instruments fluorescence lifetime imaging microscope acton sp2558
A and B show two different interactive regions acquired automatically by multiple-frequency domain <t>FLIM</t> (512x512 frame). The heat map indicates the Ef (the extent of interaction) represented by the pseudo-colour scale. A and B both show that the high expression levels of PD1, (grey scale) do not correlate with high Ef. The quantification of the Ef values is represented by violin plots next to each Ef mapped image. The heterogenous distribution of the interactive data points (per pixel) is shown by the shape of the violin plots. The dotted line at 4% Ef represents the Förster Radius (5.83nm) where there is no PD1-PDL1 interaction. PD1-PDL1 interactions are above 4% Ef.
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A and B show two different interactive regions acquired automatically by multiple-frequency domain FLIM (512x512 frame). The heat map indicates the Ef (the extent of interaction) represented by the pseudo-colour scale. A and B both show that the high expression levels of PD1, (grey scale) do not correlate with high Ef. The quantification of the Ef values is represented by violin plots next to each Ef mapped image. The heterogenous distribution of the interactive data points (per pixel) is shown by the shape of the violin plots. The dotted line at 4% Ef represents the Förster Radius (5.83nm) where there is no PD1-PDL1 interaction. PD1-PDL1 interactions are above 4% Ef.

Journal: bioRxiv

Article Title: Functional Spatial Mapping of the Tumour Immune Microenvironment In Advanced Melanoma Patients

doi: 10.1101/2025.05.29.656855

Figure Lengend Snippet: A and B show two different interactive regions acquired automatically by multiple-frequency domain FLIM (512x512 frame). The heat map indicates the Ef (the extent of interaction) represented by the pseudo-colour scale. A and B both show that the high expression levels of PD1, (grey scale) do not correlate with high Ef. The quantification of the Ef values is represented by violin plots next to each Ef mapped image. The heterogenous distribution of the interactive data points (per pixel) is shown by the shape of the violin plots. The dotted line at 4% Ef represents the Förster Radius (5.83nm) where there is no PD1-PDL1 interaction. PD1-PDL1 interactions are above 4% Ef.

Article Snippet: The objective of the computational workflow is to construct a coincident image combining data from a Lunaphore-COMET and a multiple frequency domain Fluorescence Lifetime Imaging Microscope (Lambert Instrument), for the purpose of determining spatial protein function and protein expression together.

Techniques: Expressing